Occurrence of Fusarium oxysporum strains on the coasts of Ionian Sea (Greece) and prospects for the production of oils and pigments of high-added value
Panagiotis Dritsas1, Kerasia-Vasileia Bartsoka2, ChristosAnagnostakis1,Elias Asimakis2,George Tsiamis2, George Aggelis1
1Unit of Microbiology, Section of Genetics, Cell and Developmental Biology, Department of Biology, University of Patras, 26504 Patras, Greece
2Laboratory of Systems Microbiology and Applied Genomics, Department of Sustainable Agriculture, University of Patras, 30131 Agrinio, Greece
Fusarium is a genus of cosmopolitan filamentous fungi belonging to Ascomycetes. Some species, like Fusarium oxysporum, are producers of a great number of secondary metabolites with remarkable chemical diversity and significant bioactivities. The aim of this study was to investigate the ability of eight newly isolated strains of Fusarium oxysporum from the Ionian Sea of Greece to grow under laboratory conditions and produce metabolites of high-added value, focusing on lipids and pigments production. In preliminary experiments it was shown that the strains grew well on organic media having glucose rather than glycerol as carbon source. Thus, Potato Dextrose Broth (PBD) was selected for submerged cultures in 250cc Erlenmeyer flasks. All strains gained high amounts of dry biomass (i.e., 11.3 – 11.8 g/L) at approximately 72 h of cultivation, though lipid accumulation was low (up to 2.7%, w/w). However, when the strains were cultured on a medium containing PDB at half the recommended concentration and supplemented with glucose at 40 g/L (thus having a high C:N ratio), an increased lipid accumulation was recorded (i.e., 13.0 – 30.0%, w/w), though the production of biomass was lower (i.e., 7.4 ± 0.0 – 10.1 ± 1.0 g/L). Additionally, in all cases considerable amounts of orange and red pigments were excreted in the growth medium, the production of which increased by 3 to 10 times, depending on the strain, when glucose was added to the growth medium. Lastly, the fungal cell mass contained proteins (i.e., 47.6 – 62.5, % w/w) and polysaccharides (i.e., 35.1 – 43.6 %, w/w) in significant levels. The biochemical profiles of the isolates revealed their suitability for use in various industrial applications, while these findings can give the impulse for the design of specific culture conditions which will lead to the production of these highadded value metabolites in more efficient ways for large-scale applications.
DGF – 4th ISML – Fusarium
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Posted: 20 Φεβρουαρίου, 2024 by dritsas
#2 | 4th International Symposium on Microbial Lipids
Occurrence of Fusarium oxysporum strains on the coasts of Ionian Sea (Greece) and prospects for the production of oils and pigments of high-added value
Panagiotis Dritsas1, Kerasia-Vasileia Bartsoka2, ChristosAnagnostakis1,Elias Asimakis2,George Tsiamis2, George Aggelis1
1Unit of Microbiology, Section of Genetics, Cell and Developmental Biology, Department of Biology, University of Patras, 26504 Patras, Greece
2Laboratory of Systems Microbiology and Applied Genomics, Department of Sustainable Agriculture, University of Patras, 30131 Agrinio, Greece
Fusarium is a genus of cosmopolitan filamentous fungi belonging to Ascomycetes. Some species, like Fusarium oxysporum, are producers of a great number of secondary metabolites with remarkable chemical diversity and significant bioactivities. The aim of this study was to investigate the ability of eight newly isolated strains of Fusarium oxysporum from the Ionian Sea of Greece to grow under laboratory conditions and produce metabolites of high-added value, focusing on lipids and pigments production. In preliminary experiments it was shown that the strains grew well on organic media having glucose rather than glycerol as carbon source. Thus, Potato Dextrose Broth (PBD) was selected for submerged cultures in 250cc Erlenmeyer flasks. All strains gained high amounts of dry biomass (i.e., 11.3 – 11.8 g/L) at approximately 72 h of cultivation, though lipid accumulation was low (up to 2.7%, w/w). However, when the strains were cultured on a medium containing PDB at half the recommended concentration and supplemented with glucose at 40 g/L (thus having a high C:N ratio), an increased lipid accumulation was recorded (i.e., 13.0 – 30.0%, w/w), though the production of biomass was lower (i.e., 7.4 ± 0.0 – 10.1 ± 1.0 g/L). Additionally, in all cases considerable amounts of orange and red pigments were excreted in the growth medium, the production of which increased by 3 to 10 times, depending on the strain, when glucose was added to the growth medium. Lastly, the fungal cell mass contained proteins (i.e., 47.6 – 62.5, % w/w) and polysaccharides (i.e., 35.1 – 43.6 %, w/w) in significant levels. The biochemical profiles of the isolates revealed their suitability for use in various industrial applications, while these findings can give the impulse for the design of specific culture conditions which will lead to the production of these highadded value metabolites in more efficient ways for large-scale applications.
DGF – 4th ISML – Fusarium
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